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Specific cytokine conditions selected for clones from common progenitors. After marked MSC cultures grew out from UCPs, we investigated the influence of cytokines on MSC functionality. To follow clonal selection, we transduced MSC progenitors in umbilical cord pieces (UCPs) with vectors encoding fluorescent proteins and genetic barcodes. However, ex vivo expansion is required to reach clinically relevant cell numbers, which might lead to selection of clones with different characteristics. FlowJo 10.7.Mesenchymal stromal cells (MSCs) are used in many clinical applications.
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